New Discover for Somatic Cell Reprograming

Induced pluripotent stem cell by GV oocytes extract: GVIPS cells (NEW DISCOVER FOR CELL REPROGRAMMING)

Genomic reprogramming factors in the cytoplasm of mature oocytes could be reprogrammed somatic cell cells to totipotency cells and full-term development (cloned animals). Since then, this technique has been considered an important toot not only for animal reproduction but also for regenerative medicine, genome preservation, and for study of genes function and cell biology. Moreover, in order to produce nuclear transfer ES (ntES) cells using somatic cell nuclear transfer (SCNT) for human diseases therapy, the SCNT technique requires donated fresh oocytes, which raises ethical problems for production in human cloned embryo. For this reason, the use of induced pluripotent stem (iPS) cells for genomic reprogramming and for regenerative medicine is currently a hot topic in this field. However, the use of iPS cells for human therapy by the technique of retroviruses used to insert the genes into iPS cells could cause tumors in tissues grown from the host iPS cells. Recently, Prof. Van Thuan Nguyen's Lab had found that genomic reprogramming factors in the cytoplasm of pig germinal vesicle (GV)-stage oocytes has been shown to improve the efficiency of producing cloned mouse offspring and could reprogram pig fibroblasts to stem-like cells. In this talk I will discuss whether pig GV cytoplasmic extract could induce pluripotent stem cells from mouse fibroblast (interspecies). We first established stem-like cells from mouse fibroblast cells treated with GV extracted (gviPS cells). We demonstrated that reactivation of Oct4 promoter in mouse Oct4-GFP fibroblast cell at day 10 after treated with pig GV cytoplamic extract and the formation of colonies is observed at 3 weeks after treatment. In addition, mouse gviPS cells reprogrammed with pig GV cytoplasmic extract can in vitro redifferentiate into neuron-like cells. Interestingly, gviPS cells injected into embryos of different mouse strain could be produced chimeric mice with germ line transmission. This study is published on Development  http://www.ncbi.nlm.nih.gov/pubmed/23132243.

References:

  1. Epigenetic reprogramming in somatic cells induced by extract from germinal vesicle stage pig oocytes.
    Hong-Thuy Bui, Deug-Nam Kwon, Min-Hui Kang, Mi-Hye Oh, Mi-Ryung Park, Woo-Jin Park, Seung-Sam Paik, Nguyen Van Thuan, and Jin-Hoi Kim. Development 2012.
  2. The cytoplasm of mouse germinal vesicle stage oocytes can enhance somatic cell nuclear reprogramming.
    Hong-Thuy Bui, Sayaka Wakayama, Satoshi Kishigami, Jin-Hoi Kim, Nguyen Van Thuan, and Teruhiko Wakayama. Development 2008,
  3. The histone deacetylase inhibitor scriptaid enhances nascent mRNA production and rescues full-term development in cloned inbred mice. 
    Van Thuan N, Hong-Thuy Bui, Kim JH, Hikichi T, Wakayama S, Kishigami S, Mizutani E, Wakayama T. Reproduction 2009.
  4. Injection of somatic cell cytoplasm into oocytes before intracytoplasmic sperm injection impairs full-term development and increases placental weight in mice
    Van Thuan N, Wakayama S, Kishigami S, Ohta H, Hikichi T, Mizutani E, Hong-Thuy Bui, Wakayama T. Biology of Reproduction 2006.
  5. Genomic Reprogramming Errors Do Not Accumulate with Serial Recloning in the Mouse.
    Wakayama S., Kohda T., Obokata H., Tokoro M., Chong Li, Terashita Y., Mizutani E., Van Thuan N., Kishigami S., Ishino F., Wakayama T. Cell (Cell Stem Cell) 2013.
    http://www.cell.com/cell-stem-cell/abstract/S1934-5909(13)00008-8?switch=standard